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So, the single tube contains 2,5 mM MgCl2, 0,4 mM dNTP, 0,2 mM two set of mentioned primers, 1,0 U AmpliTaq Gold Polymerase and 100 ng of soy DNA. The annealing temperature of 60°C and the needed concentration of 2,5 mM MgCl 2 were used to amplify both fragments at once. The verification was done by agarose gel electrophoresis (Fig. 3). Fig.3.

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